Hotspot

rpl16

LSC · 88817-90229 · 1413 bp

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Pi 0.0135

MarkerSeek score 52.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1413
variable_sites: 19
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCGACGATTGCTAGCTCCTA	CCTACAAACCATTCGAGCGA	50.0	50.0	58.0	58.0	1875-1992 bp	1.0	80.6
rpl16_p2	ATACCGTTCTCGCGTTTGAA	CCTACAAACCATTCGAGCGA	45.0	50.0	57.9	58.0	2006-2123 bp	1.0	80.3
rpl16_p3	TACCGTTCTCGCGTTTGAAT	CCTACAAACCATTCGAGCGA	45.0	50.0	57.9	58.0	2005-2122 bp	1.0	80.3
rpl16_p4	CCGTTCTCGCGTTTGAATTC	CCTACAAACCATTCGAGCGA	50.0	50.0	58.1	58.0	2003-2120 bp	1.0	79.8
rpl16_p5	GCCAAAAATTCGCGTTCCTT	CCTACAAACCATTCGAGCGA	45.0	50.0	58.2	58.0	2082-2199 bp	1.0	79.0