Hotspot

rps15-ycf1

SSC · 131373-131750 · 378 bp

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Pi 0.0344

MarkerSeek score 59.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 378
variable_sites: 13
pi: 0.0344
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CGCAAACCCGTCTGAGATAA	GGGGTGAAGAAGGGGATAGT	50.0	55.0	58.0	58.1	1156-1157 bp	1.0	80.9
rps15-ycf1_p2	CGCAAACCCGTCTGAGATAA	TGGGGTGAAGAAGGGGATAG	50.0	55.0	58.0	58.1	1157-1158 bp	1.0	80.9
rps15-ycf1_p3	CGCAAACCCGTCTGAGATAA	GTTGCCGAGGAACAAGAAGA	50.0	50.0	58.0	58.1	1047-1048 bp	1.0	80.6
rps15-ycf1_p4	CGCAAACCCGTCTGAGATAA	TTGCCGAGGAACAAGAAGAC	50.0	50.0	58.0	58.1	1046-1047 bp	1.0	80.5
rps15-ycf1_p5	CGCAAACCCGTCTGAGATAA	AAATGGGGGTCCTCGATGTA	50.0	50.0	58.0	58.1	1003 bp	0.5	59.1