Hotspot

ndhD-psaC

SSC · 124902-125036 · 135 bp

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Pi 0.0821

MarkerSeek score 75.80

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 135
variable_sites: 11
pi: 0.0821
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	GGAACCTGCAGATATTGGCA	AAGATGTGAATCCGCCTGTC	50.0	50.0	57.9	58.0	282-283 bp	1.0	82.7
ndhD-psaC_p2	GGAACCTGCAGATATTGGCA	AACAGAAGACTGCGTTGGTT	50.0	45.0	57.9	58.0	306-307 bp	1.0	82.4
ndhD-psaC_p3	GGAACCTGCAGATATTGGCA	TTAGGGGCTGAAACAACTCG	50.0	50.0	57.9	57.8	233-234 bp	1.0	82.2
ndhD-psaC_p4	GGAACCTGCAGATATTGGCA	AGAACAGAAGACTGCGTTGG	50.0	50.0	57.9	58.1	308-309 bp	1.0	81.6
ndhD-psaC_p5	GGAACCTGCAGATATTGGCA	CCAAGAACAGAAGACTGCGT	50.0	50.0	57.9	58.1	311-312 bp	1.0	81.6