Hotspot

ndhF-rpl32

SSC · 120155-120917 · 763 bp

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Pi 0.0198

MarkerSeek score 69.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 763
variable_sites: 15
pi: 0.0198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGTATGAGCCACCCGTATTG	TGCTGTCCAATATCCCTTCC	55.0	50.0	58.1	57.0	880-882 bp	1.0	72.6
ndhF-rpl32_p2	ATAGGAACGGGAAGTGGGAT	TGCTGTCCAATATCCCTTCC	50.0	50.0	57.8	57.0	904-906 bp	1.0	72.0
ndhF-rpl32_p3	GAACGGGAAGTGGGATGAAG	TGCTGTCCAATATCCCTTCC	55.0	50.0	58.3	57.0	900-902 bp	1.0	71.5
ndhF-rpl32_p4	GATGAAGGGTATGAGCCACC	TGCTGTCCAATATCCCTTCC	55.0	50.0	57.7	57.0	887-889 bp	1.0	71.5
ndhF-rpl32_p5	GGATGAAGGGTATGAGCCAC	TGCTGTCCAATATCCCTTCC	55.0	50.0	57.7	57.0	888-890 bp	1.0	71.5