Hotspot

trnT-UGU-trnL-UAA

LSC · 52799-53613 · 815 bp

Back to Asclepias

Pi 0.0135

MarkerSeek score 65.60

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 815
variable_sites: 11
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCCATTTC	55.0	55.0	57.9	58.2	876-896 bp	1.0	78.7
trnT-UGU-trnL-UAA_p2	TAGGAAAAAGCCGGCTATCG	CCGTAGCGTCTACCCATTTC	50.0	55.0	57.8	58.2	927-947 bp	1.0	77.9
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCCATTTC	50.0	55.0	57.8	58.2	908-928 bp	1.0	77.8
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	TAAGTCCGTAGCGTCTACCC	55.0	55.0	57.9	58.3	881-901 bp	1.0	77.3
trnT-UGU-trnL-UAA_p5	TAGGAAAAAGCCGGCTATCG	TAAGTCCGTAGCGTCTACCC	50.0	55.0	57.8	58.3	932-952 bp	1.0	76.4