Hotspot

ycf1

SSC · 112291-117918 · 5628 bp

Back to Ascarina

Pi 0.0089

MarkerSeek score 44.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5628
variable_sites: 50
pi: 0.0089
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCACACATGTCCAAGTGATG	CCATAGCAAGAGATCCCTTGT	45.0	47.6	55.7	57.1	230-242 bp	1.0	53.3
ycf1_p2	GGAATCGTCCATTGCGATAT	CCATAGCAAGAGATCCCTTGT	45.0	47.6	55.8	57.1	306-318 bp	1.0	52.6
ycf1_p3	TCACACATGTCCAAGTGATG	AGAGATCCCTTGTCTAGAGCT	45.0	47.6	55.7	57.0	222-234 bp	1.0	52.5
ycf1_p4	GGAATCGTCCATTGCGATAT	AGAGATCCCTTGTCTAGAGCT	45.0	47.6	55.8	57.0	298-310 bp	1.0	51.7
ycf1_p5	GGAATCGTCCATTGCGATAT	AGCAAGAGATCCCTTGTCTAGA	45.0	45.5	55.8	58.0	302-314 bp	1.0	51.4