Hotspot

ndhD

SSC · 124699-126201 · 1503 bp

Back to Ascarina

Pi 0.00399

MarkerSeek score 53.80

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 6
pi: 0.00399
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CCGACAAAAATCCGTACTCG	TGTACAGCGAACGATTTCAA	50.0	40.0	56.9	55.7	1655-1656 bp	1.0	53.8
ndhD_p2	CCGACAAAAATCCGTACTCG	TTGTACAGCGAACGATTTCA	50.0	40.0	56.9	55.7	1656-1657 bp	1.0	53.8
ndhD_p3	CCGACAAAAATCCGTACTCG	TTTCATTGTACAGCGAACGA	50.0	40.0	56.9	55.7	1661-1662 bp	1.0	53.8
ndhD_p4	CGGGTTTTTCTGGTCAAAGTG	TGTACAGCGAACGATTTCAA	47.6	40.0	57.9	55.7	1605 bp	1.0	53.6
ndhD_p5	CGGGTTTTTCTGGTCAAAGTG	TTGTACAGCGAACGATTTCA	47.6	40.0	57.9	55.7	1606 bp	1.0	53.6