Hotspot

rpl32-ndhF

SSC · 128848-130035 · 1188 bp

Back to Ascarina

Pi 0.0129

MarkerSeek score 64.60

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1188
variable_sites: 15
pi: 0.0129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	AAAACGCTTTTATCGCAGCC	GGAACAAGCAGAAGTCCCAA	45.0	50.0	58.0	58.0	1356-1374 bp	1.0	81.8
rpl32-ndhF_p2	AAAACGCTTTTATCGCAGCC	ACAAGCAGAAGTCCCAATCC	45.0	50.0	58.0	57.8	1353-1371 bp	1.0	80.3
rpl32-ndhF_p3	GTACGTTTCTTTGGAACCGC	GGAACAAGCAGAAGTCCCAA	50.0	50.0	57.7	58.0	1282-1300 bp	1.0	79.7
rpl32-ndhF_p4	AAAACGCTTTTATCGCAGCC	AAGATTGTTTGTTGCCGTCG	45.0	45.0	58.0	57.6	1375-1393 bp	1.0	78.8
rpl32-ndhF_p5	AAAACGCTTTTATCGCAGCC	CGAAGATTGTTTGTTGCCGT	45.0	45.0	58.0	57.6	1377-1395 bp	1.0	78.8