Hotspot

ndhD-ccsA

SSC · 126202-126470 · 269 bp

Back to Ascarina

Pi 0.0706

MarkerSeek score 76.00

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 269
variable_sites: 19
pi: 0.0706
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	TCAGTTGACAAGGTGGAAGC	GCAATTGTGGCTTCTATGGG	50.0	50.0	58.0	57.1	428 bp	1.0	76.3
ndhD-ccsA_p2	TCAGTTGACAAGGTGGAAGC	GGCAATTGTGGCTTCTATGG	50.0	50.0	58.0	57.1	429 bp	1.0	76.3
ndhD-ccsA_p3	TCCTGATTTCGTTCTCTCGC	GCAATTGTGGCTTCTATGGG	50.0	50.0	57.7	57.1	450 bp	1.0	74.1
ndhD-ccsA_p4	TCCTGATTTCGTTCTCTCGC	GGCAATTGTGGCTTCTATGG	50.0	50.0	57.7	57.1	451 bp	1.0	74.1
ndhD-ccsA_p5	CCTGATTTCGTTCTCTCGCT	GCAATTGTGGCTTCTATGGG	50.0	50.0	57.7	57.1	449 bp	1.0	74.1