Hotspot

trnR-ACG-trnN-GUU

IRb · 111322-111901 · 580 bp

Back to Ascarina

Pi 0.00862

MarkerSeek score 63.30

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 580
variable_sites: 5
pi: 0.00862
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	721 bp	1.0	78.8
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	CGGTTGGCTGTTAACTGACT	55.0	50.0	57.8	58.1	691 bp	1.0	77.9
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.8	57.8	685 bp	1.0	77.7
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	672 bp	1.0	77.6
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	CTGGTCGTAGGTTCGAATCC	55.0	55.0	57.8	57.8	673 bp	1.0	77.2