Hotspot

accD-psaI

LSC · 61876-62470 · 595 bp

Back to Ascarina

Pi 0.0118

MarkerSeek score 65.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 595
variable_sites: 7
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AACTCCACGGTTTCTTTCCT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.0	58.0	736-737 bp	1.0	72.4
accD-psaI_p2	ACTCCACGGTTTCTTTCCTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.0	58.0	735-736 bp	1.0	72.4
accD-psaI_p3	CAACTCCACGGTTTCTTTCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	56.9	58.0	737-738 bp	1.0	72.1
accD-psaI_p4	AACTCCACGGTTTCTTTCCT	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.0	57.9	742-743 bp	1.0	71.7
accD-psaI_p5	ACTCCACGGTTTCTTTCCTT	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.0	57.9	741-742 bp	1.0	71.7