Hotspot

trnF-GAA-ndhJ

LSC · 50470-51144 · 675 bp

Back to Ascarina

Pi 0.0133

MarkerSeek score 64.10

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 675
variable_sites: 9
pi: 0.0133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	813-830 bp	1.0	79.9
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	785-802 bp	1.0	79.5
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	775-792 bp	1.0	78.9
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CCACGCCTGAAACGTATCTT	50.0	50.0	58.1	58.3	811-828 bp	1.0	78.2
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	CATCCACGCCTGAAACGTAT	50.0	50.0	58.1	58.3	814-831 bp	1.0	77.7