Hotspot

trnT-GGU-psbD

LSC · 33357-34801 · 1445 bp

Back to Ascarina

Pi 0.00781

MarkerSeek score 65.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1445
variable_sites: 11
pi: 0.00781
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1567-1595 bp	1.0	79.9
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1570-1598 bp	1.0	79.8
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	CAAAAACGAAACGGTCCCTC	55.0	50.0	58.1	57.6	1566-1594 bp	1.0	78.8
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	CAAAAACGAAACGGTCCCTC	50.0	50.0	58.1	57.6	1569-1597 bp	1.0	78.6
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1544-1572 bp	1.0	78.2