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trnC-GCA-petN

LSC · 28928-29827 · 900 bp

Back to Ascarina

Pi 0.0114

MarkerSeek score 66.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 900
variable_sites: 10
pi: 0.0114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	CTGCAAATCCTTTCTCCCCA	AGCGAGACTGACTGTATCCA	50.0	50.0	57.8	57.9	950-962 bp	1.0	79.8
trnC-GCA-petN_p2	TGCAAATCCTTTCTCCCCAG	AGCGAGACTGACTGTATCCA	50.0	50.0	57.8	57.9	949-961 bp	1.0	79.8
trnC-GCA-petN_p3	ACTGCAAATCCTTTCTCCCC	AGCGAGACTGACTGTATCCA	50.0	50.0	57.8	57.9	951-963 bp	1.0	79.8
trnC-GCA-petN_p4	GCAAATCCTTTCTCCCCAGT	AGCGAGACTGACTGTATCCA	50.0	50.0	57.8	57.9	948-960 bp	1.0	79.8
trnC-GCA-petN_p5	GGGGACTGCAAATCCTTTCT	AGCGAGACTGACTGTATCCA	50.0	50.0	57.8	57.9	955-967 bp	1.0	79.8