Hotspot

trnS-GCU-trnG-UCC

LSC · 8954-10097 · 1144 bp

Back to Ascarina

Pi 0.0205

MarkerSeek score 71.20

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1144
variable_sites: 23
pi: 0.0205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	AACCGAAAGACCCCTTAACT	45.0	45.0	58.0	56.4	1253-1256 bp	1.0	69.6
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	AAACCGAAAGACCCCTTAACT	45.0	42.9	58.0	57.1	1254-1257 bp	1.0	67.7
trnS-GCU-trnG-UCC_p3	CGGAGAGAGAGGGATTCGAA	AACCGAAAGACCCCTTAACT	55.0	45.0	58.3	56.4	1301-1304 bp	1.0	67.2
trnS-GCU-trnG-UCC_p4	GCTTTAGTCCACTCAGCCAT	AACCGAAAGACCCCTTAACT	50.0	45.0	57.6	56.4	1239-1242 bp	1.0	66.7
trnS-GCU-trnG-UCC_p5	AGTCCACTCAGCCATCTCTC	AACCGAAAGACCCCTTAACT	55.0	45.0	58.5	56.4	1234-1237 bp	1.0	65.8