Hotspot

psbK-psbI

LSC · 8201-8603 · 403 bp

Back to Ascarina

Pi 0.0101

MarkerSeek score 55.80

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 403
variable_sites: 4
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TCCTCACGTCCAGGATTACG	50.0	55.0	57.9	58.9	535-540 bp	1.0	73.7
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	TCCTCACGTCCAGGATTACG	45.0	55.0	58.3	58.9	532-537 bp	1.0	71.7
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	TCCTCACGTCCAGGATTACG	45.0	55.0	58.3	58.9	533-538 bp	1.0	71.7
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	TCCTCACGTCCAGGATTACG	45.0	55.0	58.3	58.9	534-539 bp	1.0	71.7
psbK-psbI_p5	TGCCAGTCATACCTGTGTTC	TCCTCACGTCCAGGATTACG	50.0	55.0	57.5	58.9	573-578 bp	1.0	70.3