Hotspot

psbA-trnK-UUU

LSC · 1316-1558 · 243 bp

Back to Ascarina

Pi 0.0247

MarkerSeek score 66.70

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 243
variable_sites: 6
pi: 0.0247
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	GTGCTTTCGCGTCTCTCTAA	GAAAGCCGTGTGCAATGAAA	50.0	45.0	58.0	57.9	395 bp	1.0	80.0
psbA-trnK-UUU_p2	TCCAGTTACAAAAGCGACCC	GAAAGCCGTGTGCAATGAAA	50.0	45.0	58.1	57.9	424 bp	1.0	79.2
psbA-trnK-UUU_p3	GTGCTTTCGCGTCTCTCTAA	ACGGTTTGGGGAGAGATCTT	50.0	50.0	58.0	58.3	366 bp	1.0	78.4
psbA-trnK-UUU_p4	GTGCTTTCGCGTCTCTCTAA	AAAATGCAAGCACGGTTTGG	50.0	45.0	58.0	58.4	377 bp	1.0	77.7
psbA-trnK-UUU_p5	TCCAGTTACAAAAGCGACCC	ACGGTTTGGGGAGAGATCTT	50.0	50.0	58.1	58.3	395 bp	1.0	77.6