Hotspot

rpoC1

LSC · 8747-11532 · 2786 bp

Back to Asarum

Pi 0.000574
MarkerSeek score 49.00
Primer available yes
Label rpoC1

Feature Metadata

label_name
rpoC1
feature_type
gene
region
LSC
length_bp
2786
variable_sites
4
pi
0.000574
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1_p1 ACGATCTTTGGCTCTGGAAC AAACCAGAGAGAGACCCGAA 50.0 50.0 57.9 58.0 2934-2937 bp 1.0 79.2
rpoC1_p2 ACGATCTTTGGCTCTGGAAC AACCAGAGAGAGACCCGAAT 50.0 50.0 57.9 57.8 2933-2936 bp 1.0 77.4
rpoC1_p3 CGATCTTTGGCTCTGGAACT AAACCAGAGAGAGACCCGAA 50.0 50.0 57.6 58.0 2933-2936 bp 1.0 76.9
rpoC1_p4 GGAAGGAAGCTTGATCGGAA AAACCAGAGAGAGACCCGAA 50.0 50.0 57.6 58.0 2872-2875 bp 1.0 76.8
rpoC1_p5 AGGAAGGAAGCTTGATCGGA AAACCAGAGAGAGACCCGAA 50.0 50.0 58.4 58.0 2873-2876 bp 1.0 76.6