Hotspot

pafI

LSC · 47770-51153 · 3384 bp

Back to Asarum

Pi 0.00774
MarkerSeek score 64.90
Primer available yes
Label pafI

Feature Metadata

label_name
pafI
feature_type
gene
region
LSC
length_bp
3384
variable_sites
46
pi
0.00774
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
pafI_p1 GCCTTCGTATGTTCTCCGTT CGGGAGAAAAGGAGGCATTT 50.0 50.0 58.0 58.2 2316-2697 bp 1.0 84.1
pafI_p2 TTGAGCTGACATCCGTTACG CGGGAGAAAAGGAGGCATTT 50.0 50.0 58.0 58.2 2182-2563 bp 1.0 84.1
pafI_p3 CCGTTACGGTCGTTCATTCT CGGGAGAAAAGGAGGCATTT 50.0 50.0 58.0 58.2 2170-2551 bp 1.0 84.1
pafI_p4 GGATTGAGCTGACATCCGTT CGGGAGAAAAGGAGGCATTT 50.0 50.0 58.0 58.2 2185-2566 bp 1.0 84.0
pafI_p5 ATGTTCTCCGTTGCTTGTGT CGGGAGAAAAGGAGGCATTT 45.0 50.0 58.0 58.2 2308-2689 bp 1.0 83.9