Hotspot

petD-rpoA

LSC · 88853-89639 · 787 bp

Back to Asarum

Pi 0.015
MarkerSeek score 60.90
Primer available yes
Label petD-rpoA

Feature Metadata

label_name
petD-rpoA
feature_type
igs
region
LSC
length_bp
787
variable_sites
20
pi
0.015
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD-rpoA_p1 TTTTTGATCGGTACCGCAGT TGAGAATTGAACATTTTCGCA 45.0 33.3 57.8 54.7 978-1042 bp 1.0 48.0
petD-rpoA_p2 TTTTTGATCGGTACCGCAGT TGGACATTCTACAGAAGGATTTCA 45.0 37.5 57.8 57.6 936-1000 bp 1.0 47.7
petD-rpoA_p3 GGTACCGCAGTAGCTCTTTG TGAGAATTGAACATTTTCGCA 55.0 33.3 58.4 54.7 969-1033 bp 1.0 46.6
petD-rpoA_p4 GTACCGCAGTAGCTCTTTGG TGAGAATTGAACATTTTCGCA 55.0 33.3 58.4 54.7 968-1032 bp 1.0 46.6
petD-rpoA_p5 CCGCAGTAGCTCTTTGGTTA TGAGAATTGAACATTTTCGCA 50.0 33.3 57.6 54.7 965-1029 bp 1.0 46.4