Hotspot

clpP1-psbB

LSC · 83117-83574 · 458 bp

Back to Asarum

Pi 0.00218
MarkerSeek score 50.10
Primer available yes
Label clpP1-psbB

Feature Metadata

label_name
clpP1-psbB
feature_type
igs
region
LSC
length_bp
458
variable_sites
2
pi
0.00218
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP1-psbB_p1 CGCACTATACGTCAACCCAA TATGCACACGATACCAAGGC 50.0 50.0 58.0 58.1 561-562 bp 1.0 80.0
clpP1-psbB_p2 CGCACTATACGTCAACCCAA GAAAGTAACCGACCGGGATC 50.0 55.0 58.0 58.1 595-596 bp 1.0 79.9
clpP1-psbB_p3 TATACGTCAACCCAAGCTGC TATGCACACGATACCAAGGC 50.0 50.0 58.0 58.1 556-557 bp 1.0 79.9
clpP1-psbB_p4 CGCACTATACGTCAACCCAA CGGTATGCACACGATACCAA 50.0 50.0 58.0 58.1 564-565 bp 1.0 79.9
clpP1-psbB_p5 TATACGTCAACCCAAGCTGC GAAAGTAACCGACCGGGATC 50.0 55.0 58.0 58.1 590-591 bp 1.0 79.7