Hotspot

accD-psaI

LSC · 69226-69848 · 623 bp

Back to Asarum

Pi 0.00128
MarkerSeek score 48.50
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
623
variable_sites
2
pi
0.00128
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 ACACGGTTTCTTTCCCTTGA CCATTGCAATTGCCGGAAAT 45.0 45.0 57.3 58.0 733 bp 1.0 74.2
accD-psaI_p2 ACACGGTTTCTTTCCCTTGAA CCATTGCAATTGCCGGAAAT 42.9 45.0 58.0 58.0 733 bp 1.0 71.9
accD-psaI_p3 ACACGGTTTCTTTCCCTTGA CCATCGGGCCTAAACAATCT 45.0 50.0 57.3 57.7 783 bp 1.0 71.6
accD-psaI_p4 AAAAGGTGTTCTGGGTGAGT CCATTGCAATTGCCGGAAAT 45.0 45.0 56.9 58.0 763 bp 1.0 71.0
accD-psaI_p5 AAAGGTGTTCTGGGTGAGTT CCATTGCAATTGCCGGAAAT 45.0 45.0 56.9 58.0 762 bp 1.0 71.0