Hotspot

ndhC-trnV-UAC

LSC · 58050-61648 · 3599 bp

Back to Asarum

Pi 0.0101
MarkerSeek score 64.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
3599
variable_sites
63
pi
0.0101
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.92
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCTCTCACTTTGTCTCGGGT GTCAGTCGTACTAGGCGTTC 50.0 55.0 58.0 57.8 2187-2777 bp 1.0 84.6
ndhC-trnV-UAC_p2 TCTCTCACTTTGTCTCGGGT CAAGAAAATGAGGGGCCCAT 50.0 50.0 58.0 58.1 2544-2997 bp 0.8 76.5
ndhC-trnV-UAC_p3 TCTCTCACTTTGTCTCGGGT AATCTCCCTGACTCATCGCT 50.0 50.0 58.0 58.2 2504-2957 bp 0.8 75.8
ndhC-trnV-UAC_p4 TCTCTCACTTTGTCTCGGGT ATCTCCCTGACTCATCGCTT 50.0 50.0 58.0 58.2 2503-2956 bp 0.8 75.8
ndhC-trnV-UAC_p5 CCAATTCCATTCAGGGCGTA CAAGAAAATGAGGGGCCCAT 50.0 50.0 57.9 58.1 2611-2746 bp 0.4 58.7