Hotspot

trnH-GUG-trnT-GGU

LSC · 35769-36397 · 629 bp

Back to Asarum

Pi 0.0151
MarkerSeek score 66.10
Primer available yes
Label trnH-GUG-trnT-GGU

Feature Metadata

label_name
trnH-GUG-trnT-GGU
feature_type
igs
region
LSC
length_bp
629
variable_sites
17
pi
0.0151
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.95
markerseek_score
66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-trnT-GGU_p1 CGGATGTAGCCAAGTGGATC GAACCGATGACTTACGCCTT 55.0 50.0 58.1 58.0 751-2604 bp 1.0 81.3
trnH-GUG-trnT-GGU_p2 TGGATCAAGGCAGTGGATTG GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 737-2590 bp 1.0 81.3
trnH-GUG-trnT-GGU_p3 GGATCAAGGCAGTGGATTGT GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 736-2589 bp 1.0 81.3
trnH-GUG-trnT-GGU_p4 GTGGATCAAGGCAGTGGATT GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 738-2591 bp 1.0 81.3
trnH-GUG-trnT-GGU_p5 CGGATGTAGCCAAGTGGATC TAAGACTGGAGCTTCGTGGA 55.0 50.0 58.1 58.1 791-2644 bp 1.0 80.7