Hotspot

trnK-UUU-psbA

LSC · 32513-32920 · 408 bp

Back to Asarum

Pi 0.00752
MarkerSeek score 57.60
Primer available yes
Label trnK-UUU-psbA

Feature Metadata

label_name
trnK-UUU-psbA
feature_type
igs
region
LSC
length_bp
408
variable_sites
5
pi
0.00752
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-psbA_p1 AAAGATGCAAGCACGGTTTG GTGCTTTCGCGTCTCTCTAA 45.0 50.0 57.9 58.0 546-598 bp 1.0 82.1
trnK-UUU-psbA_p2 TGAGGAGGGATCCTTTCTCC GTGCTTTCGCGTCTCTCTAA 55.0 50.0 57.8 58.0 528-580 bp 1.0 81.9
trnK-UUU-psbA_p3 TCTACTCCATCCGACGAGTT GTGCTTTCGCGTCTCTCTAA 50.0 50.0 57.6 58.0 485-537 bp 1.0 80.3
trnK-UUU-psbA_p4 CCGTGTGCAATGAAAGATGC GTGCTTTCGCGTCTCTCTAA 50.0 50.0 58.4 58.0 558-610 bp 1.0 80.1
trnK-UUU-psbA_p5 GCCGTGTGCAATGAAAGATG GTGCTTTCGCGTCTCTCTAA 50.0 50.0 58.4 58.0 559-611 bp 1.0 80.1