Hotspot

trnG-UCC-trnS-GCU

LSC · 23769-24939 · 1171 bp

Back to Asarum

Pi 0.0113
MarkerSeek score 69.50
Primer available yes
Label trnG-UCC-trnS-GCU

Feature Metadata

label_name
trnG-UCC-trnS-GCU
feature_type
igs
region
LSC
length_bp
1171
variable_sites
23
pi
0.0113
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.90
markerseek_score
69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnS-GCU_p1 ACGAATCACACTTTTACCACT ATTAGCAATCCGCCGCTTTA 38.1 45.0 55.5 58.0 1133-1250 bp 1.0 57.0
trnG-UCC-trnS-GCU_p2 AGAACGAATCACACTTTTACCAC ATTAGCAATCCGCCGCTTTA 39.1 45.0 57.3 58.0 1136-1253 bp 1.0 55.9
trnG-UCC-trnS-GCU_p3 GAACGAATCACACTTTTACCACT ATTAGCAATCCGCCGCTTTA 39.1 45.0 57.3 58.0 1135-1252 bp 1.0 55.9
trnG-UCC-trnS-GCU_p4 AACGAATCACACTTTTACCACT ATTAGCAATCCGCCGCTTTA 36.4 45.0 56.2 58.0 1134-1251 bp 1.0 55.0
trnG-UCC-trnS-GCU_p5 ACGAATCACACTTTTACCACT CGGAGAGAGAGGGATTCGAA 38.1 55.0 55.5 58.3 1181-1298 bp 1.0 54.6