Hotspot

atpI-atpH

LSC · 17733-18943 · 1211 bp

Back to Asarum

Pi 0.00198
MarkerSeek score 60.70
Primer available yes
Label atpI-atpH

Feature Metadata

label_name
atpI-atpH
feature_type
igs
region
LSC
length_bp
1211
variable_sites
5
pi
0.00198
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-atpH_p1 TACCTGTCATGCTCCTTGGA AACAGAAGCGGCAGAAATCA 50.0 45.0 58.0 58.1 1339-1349 bp 1.0 79.5
atpI-atpH_p2 TACCTGTCATGCTCCTTGGA GATACCTTCTACGGCTTGGC 50.0 55.0 58.0 58.1 1426-1436 bp 1.0 79.2
atpI-atpH_p3 TACCTGTCATGCTCCTTGGA CGATACCTTCTACGGCTTGG 50.0 55.0 58.0 57.9 1427-1437 bp 1.0 79.2
atpI-atpH_p4 TACCTGTCATGCTCCTTGGA CAGCAGCAATAACAGAAGCG 50.0 50.0 58.0 57.8 1349-1359 bp 1.0 79.0
atpI-atpH_p5 TTTTGCAACTTTAGCTGCGG AACAGAAGCGGCAGAAATCA 45.0 45.0 57.9 58.1 1290-1300 bp 1.0 78.8