Hotspot

psbM-petN

LSC · 2572-3533 · 962 bp

Back to Asarum

Pi 0.00686
MarkerSeek score 56.60
Primer available yes
Label psbM-petN

Feature Metadata

label_name
psbM-petN
feature_type
igs
region
LSC
length_bp
962
variable_sites
11
pi
0.00686
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-petN_p1 TGCTACTGCGTTGTTCATTCT TAGTAAGTCTCGCTTGGGCT 42.9 50.0 58.2 58.2 1124-1130 bp 1.0 70.2
psbM-petN_p2 TGCTACTGCGTTGTTCATTC TAGTAAGTCTCGCTTGGGCT 45.0 50.0 56.7 58.2 1124-1130 bp 1.0 70.1
psbM-petN_p3 TGCTACTGCGTTGTTCATTCT ATGGGGAAGAAGTGGACTCT 42.9 50.0 58.2 57.6 1063-1069 bp 1.0 68.7
psbM-petN_p4 TGCTACTGCGTTGTTCATTC ATGGGGAAGAAGTGGACTCT 45.0 50.0 56.7 57.6 1063-1069 bp 1.0 68.5
psbM-petN_p5 TTGCTACTGCGTTGTTCATTC TAGTAAGTCTCGCTTGGGCT 42.9 50.0 57.5 58.2 1125-1131 bp 1.0 67.8