primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	psbM-petN	1	TGCTACTGCGTTGTTCATTCT	TAGTAAGTCTCGCTTGGGCT	21	20	42.9	50.0	58.2	58.2	0.0	0.0	1.385036	2572	3533	1124-1130	1.000000	70.2
psbM-petN_p2	psbM-petN	2	TGCTACTGCGTTGTTCATTC	TAGTAAGTCTCGCTTGGGCT	20	20	45.0	50.0	56.7	58.2	0.0	0.0	1.402106	2572	3533	1124-1130	1.000000	70.1
psbM-petN_p3	psbM-petN	3	TGCTACTGCGTTGTTCATTCT	ATGGGGAAGAAGTGGACTCT	21	20	42.9	50.0	58.2	57.6	0.0	0.0	1.584550	2572	3533	1063-1069	1.000000	68.7
psbM-petN_p4	psbM-petN	4	TGCTACTGCGTTGTTCATTC	ATGGGGAAGAAGTGGACTCT	20	20	45.0	50.0	56.7	57.6	0.0	0.0	1.601621	2572	3533	1063-1069	1.000000	68.5
psbM-petN_p5	psbM-petN	5	TTGCTACTGCGTTGTTCATTC	TAGTAAGTCTCGCTTGGGCT	21	20	42.9	50.0	57.5	58.2	0.0	0.0	1.687506	2572	3533	1125-1131	1.000000	67.8
rpoC1_p1	rpoC1	1	ACGATCTTTGGCTCTGGAAC	AAACCAGAGAGAGACCCGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.111727	8747	11532	2934-2937	1.000000	79.2
rpoC1_p2	rpoC1	2	ACGATCTTTGGCTCTGGAAC	AACCAGAGAGAGACCCGAAT	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.340286	8747	11532	2933-2936	1.000000	77.4
rpoC1_p3	rpoC1	3	CGATCTTTGGCTCTGGAACT	AAACCAGAGAGAGACCCGAA	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.404394	8747	11532	2933-2936	1.000000	76.9
rpoC1_p4	rpoC1	4	GGAAGGAAGCTTGATCGGAA	AAACCAGAGAGAGACCCGAA	20	20	50.0	50.0	57.6	58.0	2.1	0.0	0.414374	8747	11532	2872-2875	1.000000	76.8
rpoC1_p5	rpoC1	5	AGGAAGGAAGCTTGATCGGA	AAACCAGAGAGAGACCCGAA	20	20	50.0	50.0	58.4	58.0	2.1	0.0	0.438014	8747	11532	2873-2876	1.000000	76.6
atpI-atpH_p1	atpI-atpH	1	TACCTGTCATGCTCCTTGGA	AACAGAAGCGGCAGAAATCA	20	20	50.0	45.0	58.0	58.1	0.0	1.4	0.152571	17733	18943	1339-1349	1.000000	79.5
atpI-atpH_p2	atpI-atpH	2	TACCTGTCATGCTCCTTGGA	GATACCTTCTACGGCTTGGC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.181624	17733	18943	1426-1436	1.000000	79.2
atpI-atpH_p3	atpI-atpH	3	TACCTGTCATGCTCCTTGGA	CGATACCTTCTACGGCTTGG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.184455	17733	18943	1427-1437	1.000000	79.2
atpI-atpH_p4	atpI-atpH	4	TACCTGTCATGCTCCTTGGA	CAGCAGCAATAACAGAAGCG	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.218454	17733	18943	1349-1359	1.000000	79.0
atpI-atpH_p5	atpI-atpH	5	TTTTGCAACTTTAGCTGCGG	AACAGAAGCGGCAGAAATCA	20	20	45.0	45.0	57.9	58.1	0.0	1.4	0.236520	17733	18943	1290-1300	1.000000	78.8
atpH-atpF_p1	atpH-atpF	1	AATTTACGGACTGGTCGTGG	ATTAAACCCGAAACTCCCGG	20	20	50.0	50.0	57.9	57.9	7.6	0.0	0.207263	19190	19704	639-1144	1.000000	82.0
atpH-atpF_p2	atpH-atpF	2	AATTTACGGACTGGTCGTGG	GGCCAGTGACCTAAGGAAAC	20	20	50.0	55.0	57.9	58.2	7.6	0.0	0.266443	19190	19704	614-1119	1.000000	81.6
atpH-atpF_p3	atpH-atpF	3	TCGTGGCATTAGCGCTTTTA	ATTAAACCCGAAACTCCCGG	20	20	45.0	50.0	58.3	57.9	18.7	0.0	0.390245	19190	19704	625-1130	1.000000	80.6
atpH-atpF_p4	atpH-atpF	4	AATTTACGGACTGGTCGTGG	ATGGCCAGTGACCTAAGGAA	20	20	50.0	50.0	57.9	58.3	7.6	13.0	0.420124	19190	19704	616-1121	1.000000	80.4
atpH-atpF_p5	atpH-atpF	5	TCGTGGCATTAGCGCTTTTA	GGCCAGTGACCTAAGGAAAC	20	20	45.0	55.0	58.3	58.2	18.7	0.0	0.449426	19190	19704	600-1105	1.000000	80.2
trnG-UCC-trnS-GCU_p1	trnG-UCC-trnS-GCU	1	ACGAATCACACTTTTACCACT	ATTAGCAATCCGCCGCTTTA	21	20	38.1	45.0	55.5	58.0	0.0	0.0	3.574049	23769	24939	1133-1250	1.000000	57.0
trnG-UCC-trnS-GCU_p2	trnG-UCC-trnS-GCU	2	AGAACGAATCACACTTTTACCAC	ATTAGCAATCCGCCGCTTTA	23	20	39.1	45.0	57.3	58.0	0.0	0.0	3.706765	23769	24939	1136-1253	1.000000	55.9
trnG-UCC-trnS-GCU_p3	trnG-UCC-trnS-GCU	3	GAACGAATCACACTTTTACCACT	ATTAGCAATCCGCCGCTTTA	23	20	39.1	45.0	57.3	58.0	0.0	0.0	3.706765	23769	24939	1135-1252	1.000000	55.9
trnG-UCC-trnS-GCU_p4	trnG-UCC-trnS-GCU	4	AACGAATCACACTTTTACCACT	ATTAGCAATCCGCCGCTTTA	22	20	36.4	45.0	56.2	58.0	0.0	0.0	3.814520	23769	24939	1134-1251	1.000000	55.0
trnG-UCC-trnS-GCU_p5	trnG-UCC-trnS-GCU	5	ACGAATCACACTTTTACCACT	CGGAGAGAGAGGGATTCGAA	21	20	38.1	55.0	55.5	58.3	0.0	0.0	3.854304	23769	24939	1181-1298	1.000000	54.6
rps16-trnK-UUU_p1	rps16-trnK-UUU	1	TCTTGGAAAGGGTGCTCAAC	AAAGCCGAGTACTCTACCGT	20	20	50.0	50.0	58.0	58.2	0.0	20.3	0.187163	29001	29899	999-1003	1.000000	78.8
rps16-trnK-UUU_p2	rps16-trnK-UUU	2	TCTTGGAAAGGGTGCTCAAC	AAGCCGAGTACTCTACCGTT	20	20	50.0	50.0	58.0	58.2	0.0	20.3	0.187163	29001	29899	998-1002	1.000000	78.8
rps16-trnK-UUU_p3	rps16-trnK-UUU	3	GGTGCTCAACCTACAGGAAC	AAAGCCGAGTACTCTACCGT	20	20	55.0	50.0	58.2	58.2	1.3	20.3	0.358736	29001	29899	989-993	1.000000	77.5
rps16-trnK-UUU_p4	rps16-trnK-UUU	4	GGTGCTCAACCTACAGGAAC	AAGCCGAGTACTCTACCGTT	20	20	55.0	50.0	58.2	58.2	1.3	20.3	0.358736	29001	29899	988-992	1.000000	77.5
rps16-trnK-UUU_p5	rps16-trnK-UUU	5	TCTTGGAAAGGGTGCTCAAC	TACCGTTGAGTTAGCAACCC	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.479501	29001	29899	985-989	1.000000	76.5
trnK-UUU-psbA_p1	trnK-UUU-psbA	1	AAAGATGCAAGCACGGTTTG	GTGCTTTCGCGTCTCTCTAA	20	20	45.0	50.0	57.9	58.0	0.0	5.8	0.160054	32513	32920	546-598	1.000000	82.1
trnK-UUU-psbA_p2	trnK-UUU-psbA	2	TGAGGAGGGATCCTTTCTCC	GTGCTTTCGCGTCTCTCTAA	20	20	55.0	50.0	57.8	58.0	25.5	5.8	0.203974	32513	32920	528-580	1.000000	81.9
trnK-UUU-psbA_p3	trnK-UUU-psbA	3	TCTACTCCATCCGACGAGTT	GTGCTTTCGCGTCTCTCTAA	20	20	50.0	50.0	57.6	58.0	0.0	5.8	0.436452	32513	32920	485-537	1.000000	80.3
trnK-UUU-psbA_p4	trnK-UUU-psbA	4	CCGTGTGCAATGAAAGATGC	GTGCTTTCGCGTCTCTCTAA	20	20	50.0	50.0	58.4	58.0	0.0	5.8	0.400352	32513	32920	558-610	1.000000	80.1
trnK-UUU-psbA_p5	trnK-UUU-psbA	5	GCCGTGTGCAATGAAAGATG	GTGCTTTCGCGTCTCTCTAA	20	20	50.0	50.0	58.4	58.0	0.0	5.8	0.400352	32513	32920	559-611	1.000000	80.1
psbA-trnH-GUG_p1	psbA-trnH-GUG	1	AACCGTGCAAACCTTGGTAT	GATCCACTTGGCTACATCCG	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.139214	33983	35693	1785-1903	1.000000	84.4
psbA-trnH-GUG_p2	psbA-trnH-GUG	2	AACCGTGCAAACCTTGGTAT	CAATCCACTGCCTTGATCCA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.143575	33983	35693	1799-1917	1.000000	84.4
psbA-trnH-GUG_p3	psbA-trnH-GUG	3	AACCGTGCAAACCTTGGTAT	AATCCACTGCCTTGATCCAC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.145083	33983	35693	1798-1916	1.000000	84.4
psbA-trnH-GUG_p4	psbA-trnH-GUG	4	AACCGTGCAAACCTTGGTAT	ACAATCCACTGCCTTGATCC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.145083	33983	35693	1800-1918	1.000000	84.4
psbA-trnH-GUG_p5	psbA-trnH-GUG	5	CCTCTAGACCTAGCTGCTGT	GATCCACTTGGCTACATCCG	20	20	55.0	55.0	57.9	58.1	13.4	0.0	0.194818	33983	35693	1731-1849	1.000000	84.0
trnH-GUG-trnT-GGU_p1	trnH-GUG-trnT-GGU	1	CGGATGTAGCCAAGTGGATC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.1	58.0	0.2	0.0	0.134890	35769	36397	751-2604	1.000000	81.3
trnH-GUG-trnT-GGU_p2	trnH-GUG-trnT-GGU	2	TGGATCAAGGCAGTGGATTG	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.139252	35769	36397	737-2590	1.000000	81.3
trnH-GUG-trnT-GGU_p3	trnH-GUG-trnT-GGU	3	GGATCAAGGCAGTGGATTGT	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.140760	35769	36397	736-2589	1.000000	81.3
trnH-GUG-trnT-GGU_p4	trnH-GUG-trnT-GGU	4	GTGGATCAAGGCAGTGGATT	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.140760	35769	36397	738-2591	1.000000	81.3
trnH-GUG-trnT-GGU_p5	trnH-GUG-trnT-GGU	5	CGGATGTAGCCAAGTGGATC	TAAGACTGGAGCTTCGTGGA	20	20	55.0	50.0	58.1	58.1	0.2	0.0	0.209680	35769	36397	791-2644	1.000000	80.7
pafI_p1	pafI	1	GCCTTCGTATGTTCTCCGTT	CGGGAGAAAAGGAGGCATTT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.166547	50072	50191	2316-2697	1.000000	84.1
pafI_p2	pafI	2	TTGAGCTGACATCCGTTACG	CGGGAGAAAAGGAGGCATTT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.169777	50072	50191	2182-2563	1.000000	84.1
pafI_p3	pafI	3	CCGTTACGGTCGTTCATTCT	CGGGAGAAAAGGAGGCATTT	20	20	50.0	50.0	58.0	58.2	16.5	0.0	0.173878	50072	50191	2170-2551	1.000000	84.1
pafI_p4	pafI	4	GGATTGAGCTGACATCCGTT	CGGGAGAAAAGGAGGCATTT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.190415	50072	50191	2185-2566	1.000000	84.0
pafI_p5	pafI	5	ATGTTCTCCGTTGCTTGTGT	CGGGAGAAAAGGAGGCATTT	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.193258	50072	50191	2308-2689	1.000000	83.9
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCTCTCACTTTGTCTCGGGT	GTCAGTCGTACTAGGCGTTC	20	20	50.0	55.0	58.0	57.8	0.0	8.4	0.174329	59572	59691	2187-2777	1.000000	84.6
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TCTCTCACTTTGTCTCGGGT	CAAGAAAATGAGGGGCCCAT	20	20	50.0	50.0	58.0	58.1	0.0	27.7	0.133137	59572	59691	2544-2997	0.800000	76.5
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCTCTCACTTTGTCTCGGGT	AATCTCCCTGACTCATCGCT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.216265	59572	59691	2504-2957	0.800000	75.8
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TCTCTCACTTTGTCTCGGGT	ATCTCCCTGACTCATCGCTT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.216265	59572	59691	2503-2956	0.800000	75.8
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CCAATTCCATTCAGGGCGTA	CAAGAAAATGAGGGGCCCAT	20	20	50.0	50.0	57.9	58.1	0.0	27.7	0.181270	59572	59691	2611-2746	0.400000	58.7
trnV-UAC_p1	trnV-UAC	1	GGCCAGCCACAAGTACTAAT	CTAAGCAATGCCCATCTTTGG	20	21	50.0	47.6	57.6	57.6	3.1	0.0	1.867976	61649	62374	870-885	1.000000	66.4
trnV-UAC_p2	trnV-UAC	2	TATAGACCAACCCACCGTCC	CTAAGCAATGCCCATCTTTGG	20	21	55.0	47.6	58.5	57.6	0.0	0.0	1.959528	61649	62374	891-906	1.000000	65.7
trnV-UAC_p3	trnV-UAC	3	TCTATAGACCAACCCACCGT	CTAAGCAATGCCCATCTTTGG	20	21	50.0	47.6	57.5	57.6	0.0	0.0	1.985551	61649	62374	893-908	1.000000	65.4
trnV-UAC_p4	trnV-UAC	4	GGCCAGCCACAAGTACTAAT	TAAGCAATGCCCATCTTTGG	20	20	50.0	45.0	57.6	56.4	3.1	0.0	2.065791	61649	62374	869-884	1.000000	64.8
trnV-UAC_p5	trnV-UAC	5	GGCCAGCCACAAGTACTAAT	ACTTCTAAGCAATGCCCATCT	20	21	50.0	42.9	57.6	57.3	3.1	0.0	2.079241	61649	62374	874-889	1.000000	64.7
accD-psaI_p1	accD-psaI	1	ACACGGTTTCTTTCCCTTGA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.3	58.0	0.0	26.9	0.748429	69226	69848	733-733	1.000000	74.2
accD-psaI_p2	accD-psaI	2	ACACGGTTTCTTTCCCTTGAA	CCATTGCAATTGCCGGAAAT	21	20	42.9	45.0	58.0	58.0	0.0	26.9	1.034476	69226	69848	733-733	1.000000	71.9
accD-psaI_p3	accD-psaI	3	ACACGGTTTCTTTCCCTTGA	CCATCGGGCCTAAACAATCT	20	20	45.0	50.0	57.3	57.7	0.0	0.8	1.069836	69226	69848	783-783	1.000000	71.6
accD-psaI_p4	accD-psaI	4	AAAAGGTGTTCTGGGTGAGT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	56.9	58.0	0.0	26.9	1.150543	69226	69848	763-763	1.000000	71.0
accD-psaI_p5	accD-psaI	5	AAAGGTGTTCTGGGTGAGTT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	56.9	58.0	0.0	26.9	1.150543	69226	69848	762-762	1.000000	71.0
cemA_p1	cemA	1	ACGGAGTCGATGAATGGAGT	CCTGCACGGATGTCAGATTT	20	20	50.0	50.0	58.5	58.3	0.0	0.0	0.781836	71900	72589	829-831	1.000000	74.0
cemA_p2	cemA	2	ACGGAGTCGATGAATGGAGT	ATCCGCGGATCAAATGAGTT	20	20	50.0	45.0	58.5	57.7	0.0	34.6	0.856660	71900	72589	764-766	1.000000	73.5
cemA_p3	cemA	3	CCTTGTTGTCGAACTCATGC	CCTGCACGGATGTCAGATTT	20	20	50.0	50.0	57.4	58.3	0.0	0.0	0.858719	71900	72589	870-872	1.000000	73.4
cemA_p4	cemA	4	ACGGAGTCGATGAATGGAGT	CCGCGGATCAAATGAGTTCT	20	20	50.0	50.0	58.5	58.3	0.0	24.1	0.867945	71900	72589	762-764	1.000000	73.4
cemA_p5	cemA	5	ACGGAGTCGATGAATGGAGT	TCCGCGGATCAAATGAGTTC	20	20	50.0	50.0	58.5	58.3	0.0	27.3	0.872610	71900	72589	763-765	1.000000	73.3
petA-psbJ_p1	petA-psbJ	1	GCATCTGTTACTCTGGCACA	CTGTGATTGGTTCAGTGGGT	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.375873	73762	74830	1217-1218	1.000000	78.0
petA-psbJ_p2	petA-psbJ	2	TTGGCATCTGTTACTCTGGC	CTGTGATTGGTTCAGTGGGT	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.390592	73762	74830	1220-1221	1.000000	77.9
petA-psbJ_p3	petA-psbJ	3	ACGTGTCCAAGGTTTGTTGT	CTGTGATTGGTTCAGTGGGT	20	20	45.0	50.0	58.2	57.7	0.0	0.0	0.447753	73762	74830	1245-1246	1.000000	77.4
petA-psbJ_p4	petA-psbJ	4	GCATCTGTTACTCTGGCACA	CCTGTGATTGGTTCAGTGGG	20	20	50.0	55.0	57.9	58.5	0.0	0.0	0.561094	73762	74830	1218-1219	1.000000	76.6
petA-psbJ_p5	petA-psbJ	5	TTGGCATCTGTTACTCTGGC	CCTGTGATTGGTTCAGTGGG	20	20	50.0	55.0	57.9	58.5	0.0	0.0	0.575813	73762	74830	1221-1222	1.000000	76.4
clpP1-psbB_p1	clpP1-psbB	1	CGCACTATACGTCAACCCAA	TATGCACACGATACCAAGGC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.052847	83117	83574	561-562	1.000000	80.0
clpP1-psbB_p2	clpP1-psbB	2	CGCACTATACGTCAACCCAA	GAAAGTAACCGACCGGGATC	20	20	50.0	55.0	58.0	58.1	0.0	13.4	0.069046	83117	83574	595-596	1.000000	79.9
clpP1-psbB_p3	clpP1-psbB	3	TATACGTCAACCCAAGCTGC	TATGCACACGATACCAAGGC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.070438	83117	83574	556-557	1.000000	79.9
clpP1-psbB_p4	clpP1-psbB	4	CGCACTATACGTCAACCCAA	CGGTATGCACACGATACCAA	20	20	50.0	50.0	58.0	58.1	0.0	12.7	0.071721	83117	83574	564-565	1.000000	79.9
clpP1-psbB_p5	clpP1-psbB	5	TATACGTCAACCCAAGCTGC	GAAAGTAACCGACCGGGATC	20	20	50.0	55.0	58.0	58.1	0.0	13.4	0.086636	83117	83574	590-591	1.000000	79.7
petD-rpoA_p1	petD-rpoA	1	TTTTTGATCGGTACCGCAGT	TGAGAATTGAACATTTTCGCA	20	21	45.0	33.3	57.8	54.7	29.6	0.0	4.481883	88853	89639	978-1042	1.000000	48.0
petD-rpoA_p2	petD-rpoA	2	TTTTTGATCGGTACCGCAGT	TGGACATTCTACAGAAGGATTTCA	20	24	45.0	37.5	57.8	57.6	29.6	10.4	4.545856	88853	89639	936-1000	1.000000	47.7
petD-rpoA_p3	petD-rpoA	3	GGTACCGCAGTAGCTCTTTG	TGAGAATTGAACATTTTCGCA	20	21	55.0	33.3	58.4	54.7	0.0	0.0	4.667455	88853	89639	969-1033	1.000000	46.6
petD-rpoA_p4	petD-rpoA	4	GTACCGCAGTAGCTCTTTGG	TGAGAATTGAACATTTTCGCA	20	21	55.0	33.3	58.4	54.7	0.0	0.0	4.667455	88853	89639	968-1032	1.000000	46.6
petD-rpoA_p5	petD-rpoA	5	CCGCAGTAGCTCTTTGGTTA	TGAGAATTGAACATTTTCGCA	20	21	50.0	33.3	57.6	54.7	0.0	0.0	4.689116	88853	89639	965-1029	1.000000	46.4