Hotspot

ndhF-rpl32

SSC · 105451-106360 · 910 bp

Back to Arundo

Pi 0.0282
MarkerSeek score 65.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
910
variable_sites
36
pi
0.0282
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACACATTCCCACAAGTTCCC ATCGTCGAGATTGGGAGAGT 50.0 50.0 58.0 57.9 1844-1967 bp 1.0 85.9
ndhF-rpl32_p2 CACATTCCCACAAGTTCCCA ATCGTCGAGATTGGGAGAGT 50.0 50.0 58.0 57.9 1843-1966 bp 1.0 85.9
ndhF-rpl32_p3 ACACATTCCCACAAGTTCCC TCGTTGCCGCTAGAAAATGA 50.0 45.0 58.0 57.9 1375-1515 bp 1.0 85.6
ndhF-rpl32_p4 CACATTCCCACAAGTTCCCA TCGTTGCCGCTAGAAAATGA 50.0 45.0 58.0 57.9 1374-1514 bp 1.0 85.6
ndhF-rpl32_p5 ACACATTCCCACAAGTTCCC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 1933-2056 bp 1.0 85.4