Hotspot

ndhC-trnV-UAC

LSC · 51656-52561 · 906 bp

Back to Arundo

Pi 0.0184
MarkerSeek score 67.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
906
variable_sites
25
pi
0.0184
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGCTAAAAGTCCTGAAATCCA GGTTCGAATCCGTATAGCCC 45.5 55.0 58.3 57.9 890-1025 bp 1.0 67.0
ndhC-trnV-UAC_p2 GGGCTAAAAGTCCTGAAATCCA AGCCCAATGCAGAAAAATGC 45.5 45.0 58.3 57.9 983-1118 bp 1.0 66.6
ndhC-trnV-UAC_p3 GGGCTAAAAGTCCTGAAATCC GGTTCGAATCCGTATAGCCC 47.6 55.0 56.6 57.9 890-1025 bp 1.0 66.0
ndhC-trnV-UAC_p4 GGGGCTAAAAGTCCTGAAATC GGTTCGAATCCGTATAGCCC 47.6 55.0 56.6 57.9 891-1026 bp 1.0 66.0
ndhC-trnV-UAC_p5 GGGCTAAAAGTCCTGAAATCC AGCCCAATGCAGAAAAATGC 47.6 45.0 56.6 57.9 983-1118 bp 1.0 65.6