Hotspot

trnF-GAA-ndhJ

LSC · 49416-49980 · 565 bp

Back to Arundo

Pi 0.0307
MarkerSeek score 68.60
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
565
variable_sites
26
pi
0.0307
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA TGAAAGTTGGATAGGCTGGC 50.0 50.0 58.1 57.9 676-693 bp 1.0 81.8
trnF-GAA-ndhJ_p2 TCAAATCTGGTTCCTGGCAC TGAAAGTTGGATAGGCTGGC 50.0 50.0 58.1 57.9 661-678 bp 1.0 81.6
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA ATAGGCTGGCCCTTACGTAA 50.0 50.0 58.1 58.2 666-683 bp 1.0 81.3
trnF-GAA-ndhJ_p4 TCAAATCTGGTTCCTGGCAC ATAGGCTGGCCCTTACGTAA 50.0 50.0 58.1 58.2 651-668 bp 1.0 81.1
trnF-GAA-ndhJ_p5 AATCCTCGTGTCACCAGTTC TGAAAGTTGGATAGGCTGGC 50.0 50.0 57.5 57.9 679-696 bp 1.0 78.6