Hotspot

atpI-atpH

LSC · 32935-33741 · 807 bp

Back to Arundo

Pi 0.0157
MarkerSeek score 67.20
Primer available yes
Label atpI-atpH

Feature Metadata

label_name
atpI-atpH
feature_type
igs
region
LSC
length_bp
807
variable_sites
19
pi
0.0157
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-atpH_p1 TAGCCGCAGCCTATATAGGT GCAGTACCTTGACCAACTCC 50.0 55.0 57.5 58.2 885-976 bp 1.0 79.9
atpI-atpH_p2 TAGCCGCAGCCTATATAGGT AAGCAGCAGCAATTAGTGGA 50.0 45.0 57.5 57.8 818-909 bp 1.0 79.9
atpI-atpH_p3 TAGCCGCAGCCTATATAGGT GCCAATCCAGCAGCAATAAC 50.0 50.0 57.5 57.8 840-931 bp 1.0 79.8
atpI-atpH_p4 TAGCCGCAGCCTATATAGGT ATAACGGAAGCAGCAGCAAT 50.0 45.0 57.5 58.2 825-916 bp 1.0 79.6
atpI-atpH_p5 ATTTTTGCAACGTTAGCCGC GCAGTACCTTGACCAACTCC 45.0 55.0 58.6 58.2 898-989 bp 1.0 79.4