Hotspot

trnC-GCA-rpoB

LSC · 19744-20956 · 1213 bp

Back to Arundo

Pi 0.0235
MarkerSeek score 64.90
Primer available yes
Label trnC-GCA-rpoB

Feature Metadata

label_name
trnC-GCA-rpoB
feature_type
igs
region
LSC
length_bp
1213
variable_sites
42
pi
0.0235
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-rpoB_p1 TCCAGTATCGCCAGACCTAG AATTCCGATCTTCCTCCCCA 55.0 50.0 58.0 58.1 1795-1851 bp 1.0 84.6
trnC-GCA-rpoB_p2 TCCAGTATCGCCAGACCTAG ATTCCGATCTTCCTCCCCAA 55.0 50.0 58.0 58.1 1794-1850 bp 1.0 84.6
trnC-GCA-rpoB_p3 TCCAGTATCGCCAGACCTAG TTCCGATCTTCCTCCCCAAT 55.0 50.0 58.0 58.1 1793-1849 bp 1.0 84.6
trnC-GCA-rpoB_p4 TCCAGTATCGCCAGACCTAG CCCCACCTACACAAGCAAAT 55.0 50.0 58.0 58.1 2022-2078 bp 1.0 84.4
trnC-GCA-rpoB_p5 AAGACGCGGGAATAGAGAGA CCCCACCTACACAAGCAAAT 50.0 50.0 57.9 58.1 2614-2662 bp 1.0 83.4