Hotspot

psbM-petN

LSC · 17907-18676 · 770 bp

Back to Arundo

Pi 0.0283
MarkerSeek score 72.20
Primer available yes
Label psbM-petN

Feature Metadata

label_name
psbM-petN
feature_type
igs
region
LSC
length_bp
770
variable_sites
32
pi
0.0283
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-petN_p1 AAAACAGCCAGCCAAAATGA TAGTAAGTCTCGCTTGGGCT 40.0 50.0 56.7 58.2 868-882 bp 1.0 72.6
psbM-petN_p2 AAAACAGCCAGCCAAAATGA GTAGTATGGGGGAGGAGTGG 40.0 60.0 56.7 58.3 812-826 bp 1.0 71.9
psbM-petN_p3 AAAACAGCCAGCCAAAATGA TAGTATGGGGGAGGAGTGGA 40.0 55.0 56.7 58.4 811-825 bp 1.0 71.1
psbM-petN_p4 AAACAGCCAGCCAAAATGAT TAGTAAGTCTCGCTTGGGCT 40.0 50.0 56.5 58.2 867-881 bp 1.0 70.8
psbM-petN_p5 AACAGCCAGCCAAAATGATT TAGTAAGTCTCGCTTGGGCT 40.0 50.0 56.5 58.2 866-880 bp 1.0 70.8