Hotspot

trnD-GUC-psbM

LSC · 16754-17801 · 1048 bp

Back to Arundo

Pi 0.0335
MarkerSeek score 73.50
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
1048
variable_sites
52
pi
0.0335
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 TTCAATTGGTCAGAGCACCG TCATTTTGGCTGGCTGTTTT 50.0 40.0 58.5 56.7 1213-1214 bp 1.0 70.2
trnD-GUC-psbM_p2 TTCAATTGGTCAGAGCACCG AATCATTTTGGCTGGCTGTT 50.0 40.0 58.5 56.5 1215-1216 bp 1.0 68.3
trnD-GUC-psbM_p3 TTCAATTGGTCAGAGCACCG ATCATTTTGGCTGGCTGTTT 50.0 40.0 58.5 56.5 1214-1215 bp 1.0 68.3
trnD-GUC-psbM_p4 TTCAATTGGTCAGAGCACCG TCATTTTGGCTGGCTGTTTTT 50.0 38.1 58.5 57.4 1213-1214 bp 1.0 68.1
trnD-GUC-psbM_p5 TTCAATTGGTCAGAGCACCG TTTGGCTGGCTGTTTTTACA 50.0 40.0 58.5 56.3 1209-1210 bp 1.0 67.3