Hotspot

trnE-UUC-trnY-GUA

LSC · 16201-16240 · 40 bp

Back to Arundo

Pi 0.05
MarkerSeek score 64.10
Primer available yes
Label trnE-UUC-trnY-GUA

Feature Metadata

label_name
trnE-UUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
40
variable_sites
3
pi
0.05
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnY-GUA_p1 CGCTATCGTCTAGTGATGCC CTAGATTTTTGGGCCGAGCT 55.0 50.0 58.0 58.0 225-244 bp 1.0 80.4
trnE-UUC-trnY-GUA_p2 GGCGCTATCGTCTAGTGATG CTAGATTTTTGGGCCGAGCT 55.0 50.0 58.0 58.0 227-246 bp 1.0 80.4
trnE-UUC-trnY-GUA_p3 ACGAGTGGTTGTATATGGCG CTAGATTTTTGGGCCGAGCT 50.0 50.0 57.8 58.0 243-262 bp 1.0 78.9
trnE-UUC-trnY-GUA_p4 CGCTATCGTCTAGTGATGCC TAGATTTTTGGGCCGAGCTG 55.0 50.0 58.0 58.2 224-243 bp 1.0 78.8
trnE-UUC-trnY-GUA_p5 GGCGCTATCGTCTAGTGATG TAGATTTTTGGGCCGAGCTG 55.0 50.0 58.0 58.2 226-245 bp 1.0 78.8