Hotspot

trnK-UUU-rps16

LSC · 3973-4544 · 572 bp

Back to Arundo

Pi 0.028
MarkerSeek score 68.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
572
variable_sites
24
pi
0.028
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCACTTAAAAGCCGAG GGTGCTCAACCTACAAGAACT 50.0 47.6 58.6 57.9 692-699 bp 1.0 70.7
trnK-UUU-rps16_p2 TAGTCGCACTTAAAAGCCGA GGTGCTCAACCTACAAGAACT 45.0 47.6 57.3 57.9 693-700 bp 1.0 69.4
trnK-UUU-rps16_p3 TCGCACTTAAAAGCCGAGTA GGTGCTCAACCTACAAGAACT 45.0 47.6 57.3 57.9 690-697 bp 1.0 69.4
trnK-UUU-rps16_p4 CGCACTTAAAAGCCGAGTAC GGTGCTCAACCTACAAGAACT 50.0 47.6 57.2 57.9 689-696 bp 1.0 69.0
trnK-UUU-rps16_p5 GTCGCACTTAAAAGCCGAGT GGTGCTCAACCTACAAGAACT 50.0 47.6 58.9 57.9 691-698 bp 1.0 68.4