Hotspot

rpl16

LSC · 79766-81318 · 1553 bp

Back to Arundinella

Pi 0.00765

MarkerSeek score 49.30

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1553
variable_sites: 38
pi: 0.00765
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GATTACCAGCGGCTCCTATG	TCCCATTCTGCCCAGTGATA	55.0	50.0	57.9	58.1	1846-1885 bp	1.0	82.5
rpl16_p2	GATTACCAGCGGCTCCTATG	ACCATGTCTTCCCATTCTGC	55.0	50.0	57.9	57.9	1855-1894 bp	1.0	82.3
rpl16_p3	TTATACCGTCGTCGCCTTTG	TCCCATTCTGCCCAGTGATA	50.0	50.0	58.1	58.1	1984-2023 bp	1.0	82.2
rpl16_p4	TTATACCGTCGTCGCCTTTG	ACCATGTCTTCCCATTCTGC	50.0	50.0	58.1	57.9	1993-2032 bp	1.0	82.0
rpl16_p5	CATTATACCGTCGTCGCCTT	TCCCATTCTGCCCAGTGATA	50.0	50.0	57.9	58.1	1986-2025 bp	1.0	81.9