Hotspot

rpl32-trnL-UAG

SSC · 108731-109259 · 529 bp

Back to Arundinella

Pi 0.0212

MarkerSeek score 63.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 529
variable_sites: 25
pi: 0.0212
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAGGCTTATCCCCCATACCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.8	57.9	964-993 bp	1.0	82.9
rpl32-trnL-UAG_p2	AAGGCTTATCCCCCATACCA	ATGTCCCGAAGAAACCCAAC	50.0	50.0	57.8	58.1	1277-1306 bp	1.0	82.0
rpl32-trnL-UAG_p3	AAGGCTTATCCCCCATACCA	GATCCGCACAAAAGAGTTGC	50.0	50.0	57.8	58.0	1543-1572 bp	1.0	81.9
rpl32-trnL-UAG_p4	AAGGCTTATCCCCCATACCA	CCGAAGAAACCCAACGAGAA	50.0	50.0	57.8	58.1	1272-1301 bp	1.0	81.7
rpl32-trnL-UAG_p5	AAGGCTTATCCCCCATACCA	AATGATTAGAAGAGCGGCGG	50.0	50.0	57.8	58.1	1579-1600 bp	0.888889	75.7