Hotspot

ndhF-rpl32

SSC · 107712-108550 · 839 bp

Back to Arundinella

Pi 0.0164
MarkerSeek score 62.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
839
variable_sites
41
pi
0.0164
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAGAATAGGAATTACCCAGGC TGACATCGAAGTACGTTTTT 50.0 35.0 58.0 53.2 906-915 bp 1.0 44.8
ndhF-rpl32_p2 GGAGAATAGGAATTACCCAGGC TTGACATCGAAGTACGTTTT 50.0 35.0 58.0 53.2 907-916 bp 1.0 44.8
ndhF-rpl32_p3 GGAGAATAGGAATTACCCAGGC TTTGACATCGAAGTACGTTT 50.0 35.0 58.0 53.2 908-917 bp 1.0 44.8
ndhF-rpl32_p4 GGAGAATAGGAATTACCCAGGC TTTTGACATCGAAGTACGTT 50.0 35.0 58.0 53.2 909-918 bp 1.0 44.8
ndhF-rpl32_p5 GGAGAATAGGAATTACCCAGGC TTTTTGACATCGAAGTACGT 50.0 35.0 58.0 53.2 910-919 bp 1.0 44.8