Hotspot

trnD-GUC-psbM

LSC · 17322-18385 · 1064 bp

Back to Arundinella

Pi 0.00969

MarkerSeek score 61.90

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 1064
variable_sites: 28
pi: 0.00969
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TTCAATTGGTCAGAGCACCG	TCCTTTTGACTGGCTGTTTT	50.0	40.0	58.5	55.7	1208-1257 bp	1.0	61.2
trnD-GUC-psbM_p2	AATAGGCATGCCATACACCC	TCCTTTTGACTGGCTGTTTT	50.0	40.0	57.7	55.7	1242-1268 bp	0.888889	57.4
trnD-GUC-psbM_p3	AATAGGCATGCCATACACCC	TCCTTTTGACTGGCTGTTTTT	50.0	38.1	57.7	56.5	1242-1268 bp	0.888889	55.8
trnD-GUC-psbM_p4	AATAGGCATGCCATACACCC	AATCCTTTTGACTGGCTGTT	50.0	40.0	57.7	55.4	1244-1270 bp	0.888889	55.5
trnD-GUC-psbM_p5	AATAGGCATGCCATACACCC	ATCCTTTTGACTGGCTGTTT	50.0	40.0	57.7	55.4	1243-1269 bp	0.888889	55.5