Hotspot

trnT-GGU-trnE-UUC

LSC · 16209-16671 · 463 bp

Back to Arundinella

Pi 0.0181

MarkerSeek score 62.60

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 463
variable_sites: 28
pi: 0.0181
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GAAGTCATCGGTTCGAGTCC	GCATCGACCCAGGAAGAATT	55.0	50.0	58.1	58.0	634-644 bp	1.0	85.9
trnT-GGU-trnE-UUC_p2	GAAGTCATCGGTTCGAGTCC	CTAGATTTTTGGGCCGAGCT	55.0	50.0	58.1	58.0	717-727 bp	1.0	85.1
trnT-GGU-trnE-UUC_p3	AGCCCTTTTAACTCAGTGGC	GCATCGACCCAGGAAGAATT	50.0	50.0	58.1	58.0	743-753 bp	1.0	84.9
trnT-GGU-trnE-UUC_p4	AGCCCTTTTAACTCAGTGGC	CTAGATTTTTGGGCCGAGCT	50.0	50.0	58.1	58.0	826-836 bp	1.0	84.3
trnT-GGU-trnE-UUC_p5	AGCCCTTTTAACTCAGTGGC	GAAGATTCGACCCGTCTGTC	50.0	55.0	58.1	58.1	1134-1147 bp	1.0	83.3