primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	trnK-UUU	1	GACTCCCAAGCACACGTATT	GAGCGTCCGGTGTAATTTCA	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.377568	1378	3949	2817-2829	1.000000	79.3
trnK-UUU_p2	trnK-UUU	2	TGCGGTCAATAAGGTAGGGA	GAGCGTCCGGTGTAATTTCA	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.414093	1378	3949	2979-2991	1.000000	78.9
trnK-UUU_p3	trnK-UUU	3	AAAACACCGAACCATCCGAT	GAGCGTCCGGTGTAATTTCA	20	20	45.0	50.0	57.8	58.3	0.0	0.0	0.477008	1378	3949	2954-2966	1.000000	78.4
trnK-UUU_p4	trnK-UUU	4	GACTCCCAAGCACACGTATT	AGCGTCCGGTGTAATTTCAT	20	20	50.0	45.0	57.9	57.6	0.0	0.0	0.491352	1378	3949	2816-2828	1.000000	78.4
trnK-UUU_p5	trnK-UUU	5	AAGACGGTTTTCGGTGCTAG	GAGCGTCCGGTGTAATTTCA	20	20	50.0	50.0	58.2	58.3	0.0	0.0	0.490800	1378	3949	2931-2943	1.000000	78.3
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5588	7074	1252-1577	1.000000	84.6
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5588	7074	1263-1588	1.000000	84.1
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	58.1	57.8	8.1	16.9	0.350677	5588	7074	1256-1581	1.000000	83.5
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	20	20	50.0	55.0	58.2	57.8	0.0	16.9	0.415585	5588	7074	1267-1592	1.000000	83.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	AAACGAAATGAAATGGGGCG	20	20	45.0	45.0	58.1	57.6	8.1	0.0	0.501493	5588	7074	1313-1638	1.000000	82.3
trnG-UCC-trnM-CAU_p1	trnG-UCC-trnM-CAU	1	ACGAATCACACTTTTACCACT	GCATTACTCTGCCACTGAGT	21	20	38.1	50.0	55.5	57.6	0.0	11.2	3.929114	14117	16079	2011-2031	1.000000	51.9
trnG-UCC-trnM-CAU_p2	trnG-UCC-trnM-CAU	2	AGAACGAATCACACTTTTACCAC	GCATTACTCTGCCACTGAGT	23	20	39.1	50.0	57.3	57.6	0.0	11.2	4.061830	14117	16079	2014-2034	1.000000	50.9
trnG-UCC-trnM-CAU_p3	trnG-UCC-trnM-CAU	3	GAACGAATCACACTTTTACCACT	GCATTACTCTGCCACTGAGT	23	20	39.1	50.0	57.3	57.6	0.0	11.2	4.061830	14117	16079	2013-2033	1.000000	50.9
trnG-UCC-trnM-CAU_p4	trnG-UCC-trnM-CAU	4	AACGAATCACACTTTTACCACT	GCATTACTCTGCCACTGAGT	22	20	36.4	50.0	56.2	57.6	0.0	11.2	4.169585	14117	16079	2012-2032	1.000000	50.0
trnG-UCC-trnM-CAU_p5	trnG-UCC-trnM-CAU	5	ACGAATCACACTTTTACCACT	CTCGAACCGATGACTTCTGT	21	20	38.1	50.0	55.5	57.4	0.0	0.0	4.174398	14117	16079	2107-2127	1.000000	49.8
trnT-GGU-trnE-UUC_p1	trnT-GGU-trnE-UUC	1	GAAGTCATCGGTTCGAGTCC	GCATCGACCCAGGAAGAATT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.133435	16209	16671	634-644	1.000000	85.9
trnT-GGU-trnE-UUC_p2	trnT-GGU-trnE-UUC	2	GAAGTCATCGGTTCGAGTCC	CTAGATTTTTGGGCCGAGCT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.133435	16209	16671	717-727	1.000000	85.1
trnT-GGU-trnE-UUC_p3	trnT-GGU-trnE-UUC	3	AGCCCTTTTAACTCAGTGGC	GCATCGACCCAGGAAGAATT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.133065	16209	16671	743-753	1.000000	84.9
trnT-GGU-trnE-UUC_p4	trnT-GGU-trnE-UUC	4	AGCCCTTTTAACTCAGTGGC	CTAGATTTTTGGGCCGAGCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.133065	16209	16671	826-836	1.000000	84.3
trnT-GGU-trnE-UUC_p5	trnT-GGU-trnE-UUC	5	AGCCCTTTTAACTCAGTGGC	GAAGATTCGACCCGTCTGTC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.178255	16209	16671	1134-1147	1.000000	83.3
trnD-GUC-psbM_p1	trnD-GUC-psbM	1	TTCAATTGGTCAGAGCACCG	TCCTTTTGACTGGCTGTTTT	20	20	50.0	40.0	58.5	55.7	5.4	0.0	2.807726	17322	18385	1208-1257	1.000000	61.2
trnD-GUC-psbM_p2	trnD-GUC-psbM	2	AATAGGCATGCCATACACCC	TCCTTTTGACTGGCTGTTTT	20	20	50.0	40.0	57.7	55.7	37.2	0.0	2.623222	17322	18385	1242-1268	0.888889	57.4
trnD-GUC-psbM_p3	trnD-GUC-psbM	3	AATAGGCATGCCATACACCC	TCCTTTTGACTGGCTGTTTTT	20	21	50.0	38.1	57.7	56.5	37.2	0.0	2.830733	17322	18385	1242-1268	0.888889	55.8
trnD-GUC-psbM_p4	trnD-GUC-psbM	4	AATAGGCATGCCATACACCC	AATCCTTTTGACTGGCTGTT	20	20	50.0	40.0	57.7	55.4	37.2	0.0	2.860075	17322	18385	1244-1270	0.888889	55.5
trnD-GUC-psbM_p5	trnD-GUC-psbM	5	AATAGGCATGCCATACACCC	ATCCTTTTGACTGGCTGTTT	20	20	50.0	40.0	57.7	55.4	37.2	0.0	2.860075	17322	18385	1243-1269	0.888889	55.5
psbM-petN_p1	psbM-petN	1	AAAACAGCCAGTCAAAAGGA	GTAGTATGGGGGAGGAGTGG	20	20	40.0	60.0	55.7	58.3	0.0	0.0	2.612114	18491	19294	837-864	1.000000	63.7
psbM-petN_p2	psbM-petN	2	AAAACAGCCAGTCAAAAGGA	TAGTATGGGGGAGGAGTGGA	20	20	40.0	55.0	55.7	58.4	0.0	0.0	2.702547	18491	19294	836-863	1.000000	63.0
psbM-petN_p3	psbM-petN	3	AAAACAGCCAGTCAAAAGGA	CGCTAGTAGTATGGGGGAGG	20	20	40.0	60.0	55.7	58.5	0.0	0.0	2.783433	18491	19294	842-869	1.000000	62.3
psbM-petN_p4	psbM-petN	4	AAAAACAGCCAGTCAAAAGGA	GTAGTATGGGGGAGGAGTGG	21	20	38.1	60.0	56.5	58.3	0.0	0.0	2.819625	18491	19294	838-865	1.000000	62.0
psbM-petN_p5	psbM-petN	5	AACAGCCAGTCAAAAGGATT	GTAGTATGGGGGAGGAGTGG	20	20	40.0	60.0	55.4	58.3	0.0	0.0	2.848967	18491	19294	835-862	1.000000	61.8
trnC-GCA-rpoB_p1	trnC-GCA-rpoB	1	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	20	20	50.0	55.0	58.1	58.3	14.6	0.0	0.395580	20396	21633	1288-1353	1.000000	82.5
trnC-GCA-rpoB_p2	trnC-GCA-rpoB	2	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	20	20	50.0	55.0	58.1	58.3	14.6	0.0	0.395580	20396	21633	1287-1352	1.000000	82.5
trnC-GCA-rpoB_p3	trnC-GCA-rpoB	3	AAAGGATTTGCAGTCCCCTG	AAGTTCTTCTGCCAAGCCTT	20	20	50.0	45.0	58.1	57.6	14.6	0.0	0.442790	20396	21633	1352-1417	1.000000	82.1
trnC-GCA-rpoB_p4	trnC-GCA-rpoB	4	AAAGGATTTGCAGTCCCCTG	AGTTCTTCTGCCAAGCCTTG	20	20	50.0	50.0	58.1	58.4	14.6	0.0	0.457296	20396	21633	1351-1416	1.000000	82.0
trnC-GCA-rpoB_p5	trnC-GCA-rpoB	5	AAAGGATTTGCAGTCCCCTG	CAAGTTCTTCTGCCAAGCCT	20	20	50.0	50.0	58.1	58.4	14.6	0.0	0.457296	20396	21633	1353-1418	1.000000	82.0
atpI-atpH_p1	atpI-atpH	1	TAGCCGCAGCCTATATAGGT	CAGTACCTTGACCAACTCCG	20	20	50.0	55.0	57.5	57.9	26.3	0.0	0.607174	33718	34546	956-985	1.000000	78.1
atpI-atpH_p2	atpI-atpH	2	ATTTTTGCAACGTTAGCCGC	CAGTACCTTGACCAACTCCG	20	20	45.0	55.0	58.6	57.9	16.5	0.0	0.665908	33718	34546	969-998	1.000000	77.6
atpI-atpH_p3	atpI-atpH	3	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	20	20	50.0	45.0	57.5	57.8	26.3	3.2	0.731910	33718	34546	890-919	1.000000	77.3
atpI-atpH_p4	atpI-atpH	4	TAGCCGCAGCCTATATAGGT	GCAGTACCTTGACCAACTCC	20	20	50.0	55.0	57.5	58.2	26.3	0.0	0.717204	33718	34546	957-986	1.000000	77.2
atpI-atpH_p5	atpI-atpH	5	TAGCCGCAGCCTATATAGGT	GCCAATCCAGCAGCAATAAC	20	20	50.0	50.0	57.5	57.8	26.3	0.0	0.741275	33718	34546	912-941	1.000000	77.2
trnR-UCU-trnfM-CAU_p1	trnR-UCU-trnfM-CAU	1	AGAAGACCTCTGTCCTATCCA	TTGGGGTTACGGGTTCGAT	21	19	47.6	52.6	56.9	58.6	0.0	0.0	3.676613	38531	38697	222-222	1.000000	57.4
trnR-UCU-trnfM-CAU_p2	trnR-UCU-trnfM-CAU	2	AGGTTTAGAAGACCTCTGTCCT	TTGGGGTTACGGGTTCGAT	22	19	45.5	52.6	57.9	58.6	0.0	0.0	3.759941	38531	38697	228-228	1.000000	56.6
trnR-UCU-trnfM-CAU_p3	trnR-UCU-trnfM-CAU	3	AGAAGACCTCTGTCCTATCCA	CTTGGGGTTACGGGTTCGAT	21	20	47.6	55.0	56.9	59.7	0.0	0.0	3.800531	38531	38697	223-223	1.000000	56.4
trnR-UCU-trnfM-CAU_p4	trnR-UCU-trnfM-CAU	4	AGGTTTAGAAGACCTCTGTCCT	CTTGGGGTTACGGGTTCGAT	22	20	45.5	55.0	57.9	59.7	0.0	0.0	3.883859	38531	38697	229-229	1.000000	55.6
trnR-UCU-trnfM-CAU_p5	trnR-UCU-trnfM-CAU	5	AGAAGACCTCTGTCCTATCCA	TTGGGGTTACGGGTTCGA	21	18	47.6	55.6	56.9	58.1	0.0	0.0	4.168780	38531	38697	222-222	1.000000	53.5
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032006	48395	49251	920-969	1.000000	85.8
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.073242	48395	49251	912-961	1.000000	85.5
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.140570	48395	49251	890-939	1.000000	85.0
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.0	58.2	0.0	10.2	0.272624	48395	49251	910-959	1.000000	83.9
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATTTGAACCGATGACCCTCG	CAATCAAGTCCGTAGCGTCT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.303758	48395	49251	919-968	1.000000	83.7
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CTAACCGGGGCTAAAAGTCC	GGTTCGAATCCGTATAGCCC	20	20	55.0	55.0	58.0	57.9	6.3	0.0	0.110083	52486	53345	914-1014	1.000000	84.7
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CTAACCGGGGCTAAAAGTCC	AGCCCAATGCAGAAAAATGC	20	20	55.0	45.0	58.0	57.9	6.3	0.0	0.137641	52486	53345	1007-1107	1.000000	84.3
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CTAACCGGGGCTAAAAGTCC	CGGTTCGAATCCGTATAGCC	20	20	55.0	55.0	58.0	58.3	6.3	22.8	0.339465	52486	53345	915-1015	1.000000	82.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TAACCGGGGCTAAAAGTCCT	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	58.3	57.9	6.3	0.0	0.415737	52486	53345	913-1013	1.000000	82.3
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TAACCGGGGCTAAAAGTCCT	AGCCCAATGCAGAAAAATGC	20	20	50.0	45.0	58.3	57.9	6.3	0.0	0.443295	52486	53345	1006-1106	1.000000	81.9
atpB-rbcL_p1	atpB-rbcL	1	AGTAGTAGGATTGGTTCTCA	AGTTTCTGTTTGTGGTGACA	20	20	40.0	40.0	52.3	55.3	0.0	0.0	8.318826	56306	57113	834-852	1.000000	44.5
atpB-rbcL_p2	atpB-rbcL	2	AGTAGTAGGATTGGTTCTCAT	AGTTTCTGTTTGTGGTGACA	21	20	38.1	40.0	53.1	55.3	0.0	0.0	8.591727	56306	57113	834-852	1.000000	44.5
atpB-rbcL_p3	atpB-rbcL	3	AGTAGTAGGATTGGTTCTCA	AGTTTCTGTTTGTGGTGACAT	20	21	40.0	38.1	52.3	55.9	0.0	0.0	8.737798	56306	57113	834-852	1.000000	44.5
atpB-rbcL_p4	atpB-rbcL	4	AGTAGTAGGATTGGTTCTCA	TGCTTTAGTTTCTGTTTGTGGTG	20	23	40.0	39.1	52.3	58.0	0.0	0.0	8.662755	56306	57113	840-858	1.000000	44.4
atpB-rbcL_p5	atpB-rbcL	5	AGTAGTAGGATTGGTTCTCA	ACACCAGGTTTAAATCCAACAC	20	22	40.0	40.9	52.3	57.1	0.0	0.0	8.585261	56306	57113	863-881	1.000000	44.3
rbcL-psaI_p1	rbcL-psaI	1	TCAAATTTGAGTTCGCCCCG	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	59.1	57.9	16.9	26.9	1.198996	58545	59504	1052-1113	1.000000	74.8
rbcL-psaI_p2	rbcL-psaI	2	ATCAAATTTGAGTTCGCCCC	GTCATTGCAATTGCCGGAAA	20	20	45.0	45.0	56.7	57.9	24.2	26.9	1.403337	58545	59504	1053-1114	1.000000	73.2
rbcL-psaI_p3	rbcL-psaI	3	CAAATTTGAGTTCGCCCCG	GTCATTGCAATTGCCGGAAA	19	20	52.6	45.0	57.6	57.9	13.1	26.9	1.477292	58545	59504	1051-1112	1.000000	72.6
rbcL-psaI_p4	rbcL-psaI	4	TCAAATTTGAGTTCGCCCCG	GAAGTCATTGCAATTGCCGG	20	20	50.0	50.0	59.1	58.4	16.9	26.9	1.489162	58545	59504	1055-1116	1.000000	72.5
rbcL-psaI_p5	rbcL-psaI	5	TCAAATTTGAGTTCGCCCCG	GGAAGTCATTGCAATTGCCG	20	20	50.0	50.0	59.1	58.4	16.9	26.9	1.489162	58545	59504	1056-1117	1.000000	72.5
rpl16_p1	rpl16	1	GATTACCAGCGGCTCCTATG	TCCCATTCTGCCCAGTGATA	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.194121	79766	81318	1846-1885	1.000000	82.5
rpl16_p2	rpl16	2	GATTACCAGCGGCTCCTATG	ACCATGTCTTCCCATTCTGC	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.220843	79766	81318	1855-1894	1.000000	82.3
rpl16_p3	rpl16	3	TTATACCGTCGTCGCCTTTG	TCCCATTCTGCCCAGTGATA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.196066	79766	81318	1984-2023	1.000000	82.2
rpl16_p4	rpl16	4	TTATACCGTCGTCGCCTTTG	ACCATGTCTTCCCATTCTGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.222788	79766	81318	1993-2032	1.000000	82.0
rpl16_p5	rpl16	5	CATTATACCGTCGTCGCCTT	TCCCATTCTGCCCAGTGATA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.237890	79766	81318	1986-2025	1.000000	81.9
ndhF-rpl32_p1	ndhF-rpl32	1	GGAGAATAGGAATTACCCAGGC	TGACATCGAAGTACGTTTTT	22	20	50.0	35.0	58.0	53.2	0.0	0.0	6.789168	107712	108550	906-915	1.000000	44.8
ndhF-rpl32_p2	ndhF-rpl32	2	GGAGAATAGGAATTACCCAGGC	TTGACATCGAAGTACGTTTT	22	20	50.0	35.0	58.0	53.2	0.0	0.0	6.789168	107712	108550	907-916	1.000000	44.8
ndhF-rpl32_p3	ndhF-rpl32	3	GGAGAATAGGAATTACCCAGGC	TTTGACATCGAAGTACGTTT	22	20	50.0	35.0	58.0	53.2	0.0	0.0	6.789168	107712	108550	908-917	1.000000	44.8
ndhF-rpl32_p4	ndhF-rpl32	4	GGAGAATAGGAATTACCCAGGC	TTTTGACATCGAAGTACGTT	22	20	50.0	35.0	58.0	53.2	0.0	0.0	6.789168	107712	108550	909-918	1.000000	44.8
ndhF-rpl32_p5	ndhF-rpl32	5	GGAGAATAGGAATTACCCAGGC	TTTTTGACATCGAAGTACGT	22	20	50.0	35.0	58.0	53.2	0.0	0.0	6.789168	107712	108550	910-919	1.000000	44.8
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AAGGCTTATCCCCCATACCA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.346738	108731	109259	964-993	1.000000	82.9
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AAGGCTTATCCCCCATACCA	ATGTCCCGAAGAAACCCAAC	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.316824	108731	109259	1277-1306	1.000000	82.0
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAGGCTTATCCCCCATACCA	GATCCGCACAAAAGAGTTGC	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.253358	108731	109259	1543-1572	1.000000	81.9
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AAGGCTTATCCCCCATACCA	CCGAAGAAACCCAACGAGAA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.348763	108731	109259	1272-1301	1.000000	81.7
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AAGGCTTATCCCCCATACCA	AATGATTAGAAGAGCGGCGG	20	20	50.0	50.0	57.8	58.1	0.0	9.8	0.351381	108731	109259	1579-1600	0.888889	75.7