Hotspot

petB

LSC · 73827-75288 · 1462 bp

Back to Arundinaria

Pi 0.00319

MarkerSeek score 60.80

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1462
variable_sites: 7
pi: 0.00319
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GGTAGTTCGACCGTGGAATT	ACCACAACCTACCCGATATGA	50.0	47.6	57.6	58.2	1569-1575 bp	1.0	67.4
petB_p2	GGTAGTTCGACCGTGGAATT	ACCACAACCTACCCGATATG	50.0	50.0	57.6	56.7	1569-1575 bp	1.0	67.1
petB_p3	GGTAGTTCGACCGTGGAATT	TGAAATACCACAACCTACCCG	50.0	47.6	57.6	57.7	1575-1581 bp	1.0	66.7
petB_p4	ATTCTGGTAGTTCGACCGTG	ACCACAACCTACCCGATATGA	50.0	47.6	57.4	58.2	1574-1580 bp	1.0	65.2
petB_p5	CATTCTGGTAGTTCGACCGT	ACCACAACCTACCCGATATGA	50.0	47.6	57.4	58.2	1575-1581 bp	1.0	65.2