Hotspot

rpl32-trnL

SSC · 108326-109028 · 703 bp

Back to Arundinaria

Pi 0.0114

MarkerSeek score 42.30

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 703
variable_sites: 12
pi: 0.0114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	AAGGGTTTTTCTGGGCAACA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.1	57.9	775-794 bp	1.0	79.2
rpl32-trnL_p2	AGGGTTTTTCTGGGCAACAA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.1	57.9	774-793 bp	1.0	79.2
rpl32-trnL_p3	AAGGGTTTTTCTGGGCAACA	AGCAGCGTGTCTACCAATTT	45.0	45.0	58.1	57.8	768-787 bp	1.0	78.7
rpl32-trnL_p4	AGGGTTTTTCTGGGCAACAA	AGCAGCGTGTCTACCAATTT	45.0	45.0	58.1	57.8	767-786 bp	1.0	78.7
rpl32-trnL_p5	AGGGTTTTTCTGGGCAACAA	GCTTGAGCACTGCTTCCTAA	45.0	50.0	58.1	58.2	788-807 bp	1.0	78.7