Hotspot

ndhF-rpl32

SSC · 107323-108145 · 823 bp

Back to Arundinaria

Pi 0.0147
MarkerSeek score 63.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
823
variable_sites
18
pi
0.0147
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TCCAAAGATTTTTACGAATACGCT 55.0 33.3 58.1 57.3 927-1000 bp 1.0 43.5
ndhF-rpl32_p2 GGAAGTGGGAGAAGAGGGAT TGACATCGAAGTACGTTTTT 55.0 35.0 58.1 53.2 899-972 bp 1.0 43.3
ndhF-rpl32_p3 GGAAGTGGGAGAAGAGGGAT TTGACATCGAAGTACGTTTT 55.0 35.0 58.1 53.2 900-973 bp 1.0 43.3
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTTGACATCGAAGTACGTTT 55.0 35.0 58.1 53.2 901-974 bp 1.0 43.3
ndhF-rpl32_p5 GGAAGTGGGAGAAGAGGGAT TTTTGACATCGAAGTACGTT 55.0 35.0 58.1 53.2 902-975 bp 1.0 43.3