Hotspot

trnT-trnL

LSC · 48343-49168 · 826 bp

Back to Arundinaria

Pi 0.00565

MarkerSeek score 63.90

Primer available yes

Label trnT-trnL

Feature Metadata

label_name: trnT-trnL
feature_type: igs
region: LSC
length_bp: 826
variable_sites: 7
pi: 0.00565
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-trnL_p1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.0	58.0	919-933 bp	1.0	81.3
trnT-trnL_p2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.1	58.0	911-925 bp	1.0	81.0
trnT-trnL_p3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	55.0	50.0	57.9	58.0	889-903 bp	1.0	80.5
trnT-trnL_p4	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.0	58.2	909-923 bp	1.0	79.4
trnT-trnL_p5	ATTTGAACCGATGACCCTCG	CAATCAAGTCCGTAGCGTCT	50.0	50.0	58.0	57.7	918-932 bp	1.0	79.1