Hotspot

trnR-trnfM

LSC · 38460-38612 · 153 bp

Back to Arundinaria

Pi 0.0132

MarkerSeek score 62.10

Primer available yes

Label trnR-trnfM

Feature Metadata

label_name: trnR-trnfM
feature_type: igs
region: LSC
length_bp: 153
variable_sites: 3
pi: 0.0132
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-trnfM_p1	AGAAGACCTCTGTCCTATCCA	GTTTGGTAGCTCACAAGGCT	47.6	50.0	56.9	58.1	237-239 bp	1.0	64.5
trnR-trnfM_p2	AGAAGACCTCTGTCCTATCCA	TTTGGTAGCTCACAAGGCTC	47.6	50.0	56.9	57.8	236-238 bp	1.0	63.8
trnR-trnfM_p3	AGAAGACCTCTGTCCTATCCA	TTATCCACTAGTTCCCCGCT	47.6	50.0	56.9	57.8	280-282 bp	1.0	63.7
trnR-trnfM_p4	AGGTTTAGAAGACCTCTGTCCT	GTTTGGTAGCTCACAAGGCT	45.5	50.0	57.9	58.1	243-245 bp	1.0	63.7
trnR-trnfM_p5	AGGTTTAGAAGACCTCTGTCCT	TTATCCACTAGTTCCCCGCT	45.5	50.0	57.9	57.8	286-288 bp	1.0	63.0