Hotspot

trnD-psbM

LSC · 17554-18543 · 990 bp

Back to Arundinaria

Pi 0.00875
MarkerSeek score 52.30
Primer available yes
Label trnD-psbM

Feature Metadata

label_name
trnD-psbM
feature_type
igs
region
LSC
length_bp
990
variable_sites
13
pi
0.00875
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-psbM_p1 TTCAATTGGTCAGAGCACCG TGCGAGAATATTGACTTCCA 50.0 40.0 58.5 54.5 1074-1075 bp 1.0 49.3
trnD-psbM_p2 TAGGTATGCCATACACCCCG TGCGAGAATATTGACTTCCA 55.0 40.0 58.7 54.5 1106-1107 bp 1.0 47.8
trnD-psbM_p3 TTCAATTGGTCAGAGCACCG ATGCGAGAATATTGACTTCCA 50.0 38.1 58.5 55.1 1075-1076 bp 1.0 46.2
trnD-psbM_p4 TTCAATTGGTCAGAGCACCG TGCGAGAATATTGACTTCCAT 50.0 38.1 58.5 55.1 1074-1075 bp 1.0 46.2
trnD-psbM_p5 GTTCAATTGGTCAGAGCACC TGCGAGAATATTGACTTCCA 50.0 40.0 57.0 54.5 1075-1076 bp 1.0 45.1